https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 20α-Hydroxysteroid Dehydrogenase Expression in the Human Myometrium at Term and Preterm Birth: Relationships to Fetal Sex and Maternal Body Mass Index https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49862 Wed 28 Feb 2024 15:09:16 AEDT ]]> The Regulation of Uterine Function During Parturition: An Update and Recent Advances https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39583 Wed 27 Jul 2022 10:45:46 AEST ]]> PTGS2 (Prostaglandin Endoperoxide Synthase-2) expression in term human amnion in vivo involves rapid mRNA turnover, polymerase-II 5'-pausing, and glucocorticoid transrepression https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15716 Wed 11 Apr 2018 17:09:52 AEST ]]> How does progesterone relax the uterus in pregnancy? https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16696 Wed 11 Apr 2018 16:57:04 AEST ]]> Effects of fetal sex on expression of the (pro)renin receptor and genes influenced by its interaction with prorenin in human amnion https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21815 ATP6AP2) would act in a fetal sex-specific manner in human amnion to regulate the expression of promyelocytic zinc finger, a negative regulator of ATP6AP2 expression as well as 2 pathways that might influence the onset of labor, namely transforming growth factor ß1 (TGFB1) and prostaglandin endoperoxide synthase 2 (PTGS2). Our findings demonstrate that there are strong interactions between prorenin, ATP6AP2, and TGFB1 and that this system has a greater capacity in female amnion to stimulate profibrotic pathways, thus maintaining the integrity of the fetal membranes. In contrast, glucocorticoids or other factors independent of the prorenin/prorenin receptor pathway may be important regulators of PTGS2 in human pregnancy.]]> Wed 11 Apr 2018 16:47:12 AEST ]]> Mechanisms leading to increased risk of preterm birth in growth-restricted guinea pig pregnancies https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16518 62 days) and labor. The PTGS1 expression was significantly upregulated in the myometrium of IUGR animals, and chorionic HPGD expression was markedly decreased (P < .01 and P < .001, respectively). These findings suggest a shift in the balance of PG production over metabolism in IUGR pregnancies leads to a greater susceptibility to preterm birth.]]> Wed 11 Apr 2018 16:46:29 AEST ]]> Progesterone receptor expression declines in the guinea pig uterus during functional progesterone withdrawal and in response to prostaglandins https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16535 Wed 11 Apr 2018 16:27:14 AEST ]]> Molecular evidence of a (pro)renin/(pro)renin receptor system in human intrauterine tissues in pregnancy and its association with PGHS-2 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16501 Wed 11 Apr 2018 16:26:57 AEST ]]> DNA methylation in development https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:13745 Wed 11 Apr 2018 16:02:31 AEST ]]> Progesterone receptor isoform expression in the guinea pig myometrium from normal and growth restricted pregnancies https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10509 Wed 11 Apr 2018 15:35:47 AEST ]]> Modulation of progesterone receptor isoform expression in pregnant human myometrium https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30926 PR isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise PR isoform expression in culture. Methods: Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF2α, NF-κB inhibitors, or TSA. Following 48 h culture, PR-T, PR-A, and PR-B mRNA levels were determined using qRT-PCR. PR-A/PR-B ratios were calculated. Results: PR-T and PR-A expression and the PR-A/PR-B ratio significantly increased in culture. Steroids prevented the culture-induced increase in PR-T and PR-A expression. Stretch blocked the effects of steroids on PR-T and PR-A expression. PMA further increased the PR-A/PR-B ratio, while TSA blocked culture-induced increases of PR-A expression and the PR-A/PR-B ratio. Conclusion: Human myometrial tissue in culture undergoes changes in PR gene expression consistent with transition toward a laboring phenotype. TSA maintained the nonlaboring PR isoform expression pattern. This suggests that preserving histone and/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture.]]> Wed 11 Apr 2018 14:38:10 AEST ]]> Inflammatory and steroid receptor gene methylation in the human amnion and decidua https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16515 Wed 11 Apr 2018 13:10:22 AEST ]]> Fetal sex affects expression of renin-angiotensin system components in term human decidua https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15206 Wed 11 Apr 2018 11:04:16 AEST ]]> Expression of glucocorticoid receptor messenger ribonucleic acid transcripts in the human placenta at term https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:4591 Wed 11 Apr 2018 10:27:42 AEST ]]> The expression of genes involved in myometrial contractility changes during ex situ culture of pregnant human uterine smooth muscle tissue https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34255 Ex situ analyses of human myometrial tissue has been used to investigate the regulation of uterine quiescence and transition to a contractile phenotype. Following concerns about the validity of cultured primary cells, we examined whether myometrial tissue undergoes culture-induced changes ex situ that may affect the validity of in vitro models. Objectives: To determine whether human myometrial tissue undergoes culture-induced changes ex situ in Estrogen receptor 1 (ESR1), Prostaglandin-endoperoxide synthase 2 (PTGS2) and Oxytocin receptor (OXTR) expression. Additionally, to determine whether culture conditions approaching the in vivo environment influence the expression of these key genes. Methods: Term non-laboring human myometrial tissues were cultured in the presence of specific treatments, including; serum supplementation, progesterone and estrogen, cAMP, PMA, stretch or NF-κB inhibitors. ESR1, PTGS2 and OXTR mRNA abundance after 48 h culture was determined using quantitative RT-PCR. Results: Myometrial tissue in culture exhibited culture-induced up-regulation of ESR1 and PTGS2 and down-regulation of OXTR mRNA expression. Progesterone prevented culture-induced increase in ESR1 expression. Estrogen further up-regulated PTGS2 expression. Stretch had no direct effect, but blocked the effects of progesterone and estrogen on ESR1 and PTGS2 expression. cAMP had no effect whereas PMA further up-regulated PTGS2 expression and prevented decline of OXTR expression. Conclusion: Human myometrial tissue in culture undergoes culture-induced gene expression changes consistent with transition toward a laboring phenotype. Changes in ESR1, PTGS2 and OXTR expression could not be controlled simultaneously. Until optimal culture conditions are determined, results of in vitro experiments with myometrial tissues should be interpreted with caution.]]> Wed 04 Sep 2019 10:05:05 AEST ]]> Epigenetic regulation of progesterone receptors and the onset of labour https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:47796 Tue 31 Jan 2023 15:32:49 AEDT ]]> Preterm labor is a distinct process from term labor following computational analysis of human myometrium https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44767 Tue 30 May 2023 11:48:47 AEST ]]> Mammalian labor: variations on a theme by amniota https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:13863 Tue 24 Aug 2021 14:21:59 AEST ]]> Promoter methylation pattern controls corticotropin releasing hormone gene activity in human trophoblasts https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30964 100) epialleles. RNA-polymerase-II (Pol-II) bound only to three particular epialleles in cAMP-stimulated cells, while phospho-cAMP response element-binding protein (pCREB) bound to only one epiallele, which was different from those selected by Pol-II. Binding of TATA-binding protein increased during syncytial differentiation preferentially at epialleles compatible with Pol-II and pCREB binding. Histone-3 acetylation was detected only at epialleles targeted by Pol-II and pCREB, while gene activating histone-4 acetylation and histone-3-lysine-4 trimethylation occurred at CRH epialleles not associated with Pol-II or pCREB. The suppressive histone-3-lysine-27 trimethyl and-lysine-9 trimethyl modifications showed little or no epiallele preference. The epiallele selectivity of activating histone modifications and transcription factor binding demonstrates the epigenetic and functional diversity of the CRH gene in trophoblasts, which is controlled predominantly by the patterns, not the overall extent, of promoter methylation. We propose that conditions impacting on epiallele distribution influence the number of transcriptionally active CRH gene copies in the trophoblast cell population determining the gestational trajectory of placental CRH production in normal and pathological pregnancies.]]> Tue 24 Apr 2018 15:36:22 AEST ]]> Methylation of promoter regions of genes of the human intrauterine renin angiotensin system and their expression https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:26759 Tue 24 Apr 2018 15:35:25 AEST ]]> Methylation of the corticotropin releasing hormone gene promoter in BeWo cells: relationship to gene activity https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:22723 Tue 24 Apr 2018 15:33:43 AEST ]]> Birth and neonatal transition in the guinea pig: experimental approaches to prevent preterm birth and protect the premature fetus https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35228 in utero. This allows adverse intrauterine conditions to make a sustained impact on the developing brain like in compromised human pregnancies. In addition, the brain is exposed to a protective neurosteroid environment in utero, which has been suggested to promote development in the guinea pig and the human. Moreover, in utero stresses that have been shown to adversely affect long term neurobehavioral outcomes in clinical studies, can be modeled successfully in guinea pigs. Overall, these parallels to the human have led to increasing interest in the guinea pig for translational studies of treatments and therapies that potentially improve outcomes following adverse events in pregnancy and after preterm birth.]]> Tue 02 Jul 2019 11:37:43 AEST ]]> Regulation of 20α-Hydroxysteroid Dehydrogenase Expression in Term Pregnant Human Myometrium Ex Vivo https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54561 Thu 29 Feb 2024 12:33:44 AEDT ]]> Regulation of inflammatory genes in decidual cells: Involvement of the bromodomain and extra-terminal family proteins https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52773 Thu 26 Oct 2023 14:54:24 AEDT ]]> Preterm labor with and without chorioamnionitis is associated with activation of myometrial inflammatory networks: a comprehensive transcriptomic analysis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51190 Thu 24 Aug 2023 14:39:18 AEST ]]> Genes upregulated in the amnion at labour are bivalently marked by activating and repressive histone modifications https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41699 PTGS2, BMP2 and NAMPT was determined by reverse transcription-coupled quantitative real-Time PCR (qRT-PCR). Chromatin immunoprecipitation (ChIP) and sequential double ChIP were performed to determine the levels and co-occurrence of activating histone-3, lysine-4 trimethylation (H3K4me3) and repressive histone-3, lysine-27 trimethylation (H3K27me3) at the gene promoters. H3K4 methyltransferase, H3K27me3 demethylase and H3K27 methyltransferase expression was determined by qRT-PCR and immunofluorescence confocal microscopy. PTGS2, BMP2 and NAMPT expression was upregulated robustly between early pregnancy and term (P < 0.05). The promoters were marked bivalently by both the H3K4me3 and H3K27me3 modifications. Bivalence was reduced at term by the decrease of the H3K27me3-modified fraction of promoter copies marked by H3K4me3 indicating epigenetic activation. Messenger RNAs encoding the H3K4-specific methyl transferases MLL1,-2,-3,-4, SETD1A,-B and the H3K27me3-specific demethylases KDM6A,-B were expressed increasingly while the H3K27 methyl transferase EZH2 was expressed decreasingly at term. Histone modifying enzyme proteins were detected in amnion epithelial and mesenchymal cells. These results with prototypical proinflammatory genes suggest that nucleosomes at labour-promoting genes are marked bivalently in the amnion, which is shifted towards monovalent H3K4me3 modification at term when the genes are upregulated. Bivalent epigenetic regulation by histone modifying enzymes may control the timing of labour.]]> Thu 11 Aug 2022 14:00:32 AEST ]]> Nuclear progesterone receptor expression in the human fetal membranes and decidua at term before and after labor https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:7827 Sat 24 Mar 2018 08:37:39 AEDT ]]> Prostaglandin H₂ Synthase-1 and -2 expression in guinea pig gestation tissues during late pregnancy and parturition https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1957 Sat 24 Mar 2018 08:33:18 AEDT ]]> Reduced 11β-hydroxysteroid dehydrogenase type 2 activity is associated with decreased birth weight centile in pregnancies complicated by asthma https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1476 Sat 24 Mar 2018 08:28:11 AEDT ]]> Regulation of 15-hydroxyprostaglandin dehydrogenase (PGDH) gene activity, messenger ribonucleic acid processing, and protein abundance in the human chorion in late gestation and labour https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:2467 Sat 24 Mar 2018 08:27:48 AEDT ]]> Maternal asthma is associated with reduced female fetal growth https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:1792 Sat 24 Mar 2018 08:27:30 AEDT ]]> The control of prostaglandin endoperoxide H-synthase-2 expression in the human chorion laeve at term https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:2296 Sat 24 Mar 2018 08:26:56 AEDT ]]> 15-hydroxyprostaglandin dehydrogenase expression and localization in guinea pig gestational tissues during late pregnancy and parturition https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16526 Sat 24 Mar 2018 08:01:18 AEDT ]]> Estrogen receptor (ER) expression and function in the pregnant human myometrium: estradiol via ERa activates ERK1/2 signaling in term myometrium https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:18810 Sat 24 Mar 2018 07:51:01 AEDT ]]> Separation of bioactive prostaglandins and their metabolites by reversed-phase thin-layer chromatography https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:3474 Sat 24 Mar 2018 07:20:27 AEDT ]]> Expression of KCNH2 (hERG1) and KCNE2 correlates with expression of key myometrial genes in term pregnant human myometrium https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:32092 Mon 23 Sep 2019 12:35:23 AEST ]]> Histone deacetylase inhibitors: providing new insights and therapeutic avenues for unlocking human birth https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:49910 Mon 19 Jun 2023 11:07:36 AEST ]]> The intrauterine renin-angiotensin system: sex-specific effects on the prevalence of spontaneous preterm birth https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34341 Mon 04 Mar 2019 14:57:46 AEDT ]]> Fetal membrane epigenetics https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:39927 Fri 15 Jul 2022 10:34:45 AEST ]]> Perinatal compromise contributes to programming of GABAergic and glutamatergic systems leading to long-term effects on offspring behaviour https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37890 Fri 11 Jun 2021 09:29:09 AEST ]]>